相比较其他检测技术,高通量测序技术具有高效、快速等特点,并拥有全面分析复杂核苷酸群体的功能,本文运用高通量测序技术对进境澳大利亚4批次小麦种子和筛下物上的病原真菌进行检测,通过基因组DNA提取、高通量测序、生物信息学分析后得到分类OTU共622个,基于不同分类水平,分属于5个门、64个目、201个属。检测显示,种子与筛下物携带的病原真菌的种类和比例有较大差异,并且筛下物携带的病原真菌数量和种类远多于种子携带的病原真菌。 Compared with other detection technologies, high-throughput sequencing technology has the characteristics of high efficiency and rapidity, and has a comprehensive analysis of the function of complex nucleotide populations. In this paper, high-throughput sequencing technology was applied to four batches of wheat seeds and undersize objects The pathogenic fungi were detected by genomic DNA extraction, high-throughput sequencing and bioinformatics analysis. The total number of classified OTUs was 622, belonging to 5 phylum, 64 orders and 201 genera based on different taxonomic levels. The results showed that the species and proportion of pathogenic fungi carried by the seeds and the undersize were quite different, and the number and types of pathogenic fungi carried by the undersize were far more than the pathogenic fungi carried by the seeds.