推断为产气荚膜杆菌引起的散发腹泻的细菌学探讨

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以入院或门诊的散发腹泻患者88例为对象,检查粪便的产气荚膜杆菌,并应用反向被动血凝试验检查产气荚膜杆菌的肠毒素,对本菌引起的散发腹泻进行了调查。从3例腹泻患者粪便中以纯培养状态检出了产气荚膜杆菌,而且在粪便中证明了肠毒素,因此这3例被推断为产气荚膜杆菌性肠炎。粪便中的肠毒素量(反向被动血凝价)2例为1∶640,另1例为1∶5,120。这3例粪便中的产气荚膜杆菌数都是10~8个/g。分离菌株的血清型不符合Hobbs血清型,符合作者们的TW血清型23、47及57。用Duncan & strong培养基及Tortora培养基探讨的结果是,TW血清型47和57两株在培养液中产生了肠毒素,可是TW血清型23在两培养基上,其芽胞形成情况显著不佳,肠毒素为阴性。从其他85例腹泻粪便中没有检出肠毒素。其中29例检出产气荚膜杆菌,其菌量与健康者相似,为10~5个/g以下。关于产气荚膜杆菌引起腹泻,只是对较易检出细菌的大规模集体食物中毒做过详细探讨;产气荚膜杆菌是健康人的肠道内常在细菌,从细菌检查结果弄不清其因果关系的散发性腹泻,几乎没被探讨过。作者们在前报报告了由产气荚膜杆菌引起的集体食物中毒的细菌学诊断,历来的细菌检查结合患者粪便的肠毒素检查是极为有效的方法。应用这个方法,以入院或门诊的散发腹泻患者为对象检查粪便中的肠毒素及分离菌株的肠毒素的产生状况,得以弄清系产气荚膜杆菌引起的散发病例,所以将其结果报告于下。 88 cases of patients with diarrhea who were hospitalized or outpatient were examined for Faecium capsulatum excreted, and the enterotoxin of C. perfringens was examined by reverse passive hemagglutination test to investigate the sporadic diarrhea caused by this bacterium. C. perfringens was detected purely in the feces of 3 patients with diarrhea and proved to be enterotoxins in the stool, so these 3 cases were inferred to be C. pneumoniae enteritis. Stool in the amount of enterotoxin (reverse passive coagulation) 2 cases of 1: 640, the other 1 case was 1: 5,120. The number of Clostridium perfringens in the three cases of stool was 10 ~ 8 / g. The serotypes of the isolated isolates did not correspond to the Hobbs serotypes and corresponded to the authors’ TW serotypes 23, 47 and 57. As a result of the Duncan & strong medium and the Tortora medium, two strains of TW serotypes 47 and 57 produced enterotoxins in the culture medium, but TW serotype 23 on both mediums had significantly poor sporulation , Enterotoxin is negative. No other toxins were detected in the other 85 diarrhea cases. Among them, 29 strains of Clostridium perfringens were detected, their bacterial counts were similar to those of healthy subjects, and were below 10 to 5 / g. With regard to C. albicans causing diarrhea, it is only a detailed examination of the large-scale collective food poisoning that is more likely to be detected; C. aeruginosa is a healthy person’s gut that is often in the gut and unclear from the bacterial test results Consequential sporadic diarrhea has hardly been explored. In a previous report, the authors report on the bacteriological diagnosis of collective food poisoning caused by C. perfringens. Historically, bacterial tests have proven to be extremely effective in examining enterotoxins in patients’ feces. Using this method, the presence of enterotoxins in the stools and enterotoxins of the isolated strains was examined in patients who were hospitalized or outpatient diarrhea to clarify the cases of C. difficile-induced excretion, and the results were reported in under.
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