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目的探讨牛磺酸、维生素C联合对染铅大鼠拮抗铅以及海马中一氧化氮合成酶(NOS)活性、一氧化氮(NO)水平和学习记忆的影响。方法将体重为(85.23±8.1)g的断乳SD大鼠22只,随机分为实验组10只,对照组12只。各组SD大鼠自由饮用铅水(1.3g/L硝酸铅溶液),同时实验组大鼠用牛磺酸、维生素C混合溶液灌胃,而对照组大鼠用去离子水灌胃,每周5次,共8周。测定大鼠血铅值、海马组织中NOS活性和NO水平。用Morris水迷宫实验测试大鼠学习记忆能力。结果实验组血铅值低于对照组,差异有统计学意义(P﹤0.01);实验组海马组织中NOS活性和NO水平高于对照组,差异有统计学意义,分别是(P﹤0.01)和(P﹤0.05);Morris水迷宫测试实验组d4潜伏期短于对照组,差异有统计学意义(P﹤0.05)。结论牛磺酸、维生素C联合作用于铅暴露大鼠有排铅效果,能提高海马组织中NOS活性和NO水平并有改善学习记忆能力的作用。
Objective To investigate the effects of taurine and vitamin C on antagonism of lead and hippocampal nitric oxide synthase (NOS) activity, nitric oxide (NO) and learning and memory in lead-exposed rats. Methods Twenty-two SD rats weighing (85.23 ± 8.1) g were randomly divided into experimental group (n = 10) and control group (n = 12). The rats in each group were allowed to drink lead (1.3g / L lead nitrate solution) for free. At the same time, the rats in the experimental group were orally administered with the mixed solution of taurine and vitamin C. The rats in the control group were orally administered with deionized water every week 5 times, a total of 8 weeks. Blood lead levels, NOS activity and NO level in hippocampus were measured. Morris water maze test was used to test learning and memory abilities in rats. Results The level of blood lead in the experimental group was lower than that in the control group (P <0.01). The activity of NOS and the level of NO in the hippocampus of the experimental group were significantly higher than those in the control group (P <0.01) (P <0.05). The latency of d4 in Morris water maze test group was shorter than that in control group (P <0.05). Conclusion The combined effect of taurine and vitamin C on lead exposed rats has the effect of excreting lead, which can improve the activity of NOS and the level of NO in hippocampus and improve the learning and memory ability.