论文部分内容阅读
目的探讨宫颈癌组织中诱骗受体3(DCR3)表达与外周血T细胞亚群之间的相关性。方法免疫组织化学法检测宫颈上皮内瘤变(CIN)、宫颈癌(CC)及正常宫颈组织中DCR3表达情况,并检测宫颈癌组织中实质及间质内T细胞表面抗原CD3、CD4、CD8阳性细胞数量;应用流式细胞术分析患者外周血中CD3+、CD4+、CD8+T数量变化情况,分析研究宫颈癌组织中DCR3表达与患者外周血T细胞亚群之间的相关性。结果 DCR3表达强度与宫颈病变严重程度有关;宫颈癌患者外周血中T细胞数明显降低,且宫颈组织中DCR3表达与患者外周血中CD3+T、CD4+/CD8+呈负相关;宫颈癌变后实质内T细胞数明显减少,T细胞主要聚集于肿瘤间质内。结论 DCR3参与了宫颈癌细胞免疫逃逸,外周血T细胞亚群联合DCR3检测可作为宫颈癌免疫诊断指标,为患者免疫治疗提供基础。
Objective To investigate the correlation between decoy receptor 3 (DCR3) expression and T cell subsets in peripheral blood in cervical cancer. Methods Immunohistochemical method was used to detect the expression of DCR3 in cervical intraepithelial neoplasia (CIN), cervical cancer (CC) and normal cervical tissue. The positive and negative T cell surface antigens CD3, CD4 and CD8 in cervical cancer tissues were detected The numbers of CD3 +, CD4 +, CD8 + T cells in peripheral blood were analyzed by flow cytometry. The correlation between the expression of DCR3 and T lymphocyte subsets in peripheral blood of patients with cervical cancer was analyzed. Results The expression of DCR3 was correlated with the severity of cervical lesions. The number of T cells in peripheral blood of patients with cervical cancer was significantly lower than that in patients with cervical cancer. The expression of DCR3 in cervical tissues was negatively correlated with the levels of CD3 + T and CD4 + / CD8 + T cells decreased significantly, T cells mainly gathered in the tumor stroma. Conclusions DCR3 is involved in the immune escape of cervical cancer cells. Combined detection of peripheral blood T cell subsets and DCR3 may serve as an immunological diagnostic indicator for cervical cancer, providing a basis for immunotherapy.