棘球蚴病的免疫诊断方法主要是检测病人血清中的特异抗体,现有检测方法的敏感性和特异性仍不理想,与所采用的抗原性质有关。由于缺乏标准化的诊断抗原,不同实验室的检测结果不一,也无法比较。因此对棘球蚴囊液抗原有进一步研究必要。本文采用淋巴细胞杂交瘤技术制备了高效特异的单克隆抗体(McAb),对不同宿主的棘球蚴囊液抗原组分的理化特性和抗原性进行了分析。 以初步纯化的人源棘球蚴囊液抗原免疫BALB The immune diagnosis of hydatid disease is mainly to detect the specific antibodies in the serum of the patient. The sensitivity and specificity of the existing detection methods are still not ideal, which are related to the antigenic properties used. Due to the lack of standardized diagnostic antigen, different laboratories have different test results and can not be compared. Therefore, hydatid cyst fluid antigen further research necessary. In this paper, monoclonal antibody (McAb) with high specificity and specificity was prepared by using lymphocyte hybridoma technique. The physicochemical properties and antigenicity of hydatid cyst fluid antigen components from different hosts were analyzed. BALB was immunized with the primary purified hydatid cyst antigen