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采用原位ＰＣＲ方法在培养的人小肠癌转移腹水细胞系（ＨＩＣＭＡ）中进行了人Ｙ染色体上单拷贝ＳＲＹ基因的原位扩增，并以ＰＣＲ方法制备的地高辛标记探针原位检测了所扩增的片段，比较研究表明，原位ＰＣＲ法较之直接的原位杂交法，其灵敏度提高５倍以上
In situ PCR was used to amplify the single copy SRY gene in human Y chromosome in cultured human intestinal cancer ascites cell line (HICMA). In situ detection of digoxigenin-labeled probe by PCR The amplified fragments, comparative studies have shown that the in situ PCR method compared with direct in situ hybridization, the sensitivity increased more than 5 times