生物素 -亲和素介导的肿瘤靶向治疗是近几年来肿瘤早期诊断、治疗研究的热点。将包封有 TNFα的壳聚糖微球生物素化 ,以生物素化无唾液酸胎球蛋白为导向系统 ,采用“三步法”研究了微球体外抗肿瘤毒性实验。结果表明 :生物素化壳聚糖微球对体外培养的肝癌细胞 SMMC- 772 1有显著的抑制作用 (抑制率为 6 0 .8% ,P<0 .0 1)。 Biotin-avidin-mediated tumor-targeted therapy is a hot topic in the early diagnosis and treatment of tumors in recent years. The chitosan microspheres encapsulated with TNFα were biotinylated and the biotinylated asialofetuin-oriented system was used. The “three-step method” was used to study the in vitro antitumor toxicity of microspheres. The results showed that the biotinylated chitosan microspheres had significant inhibitory effect on the in vitro cultured hepatoma cells SMMC-772 1 (inhibition rate was 60.8%, P<0.01).