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目的:观察对乙酰氨基酚对肝细胞损伤的影响及阿魏酸钠的保护作用。方法:采用原代肝细胞培养,以细胞存活率及培养液中谷丙转氨酶(ALT)活性的变化为指标。结果:对乙酰氨基酚2μg·ml-1与细胞共同培养3h可形成稳定损伤,阿魏酸钠可明显提高损伤肝细胞活率,降低ALT活性。结论:阿魏酸钠对肝损伤有保护作用。
Objective: To observe the effect of acetaminophen on hepatocellular injury and the protective effect of sodium ferulate. Methods: Primary hepatocytes were cultured and the changes of cell viability and ALT activity in the culture medium were taken as indexes. Results: Paracetamol 2μg · ml-1 co-cultured with cells for 3h to form a stable injury, sodium ferulate can significantly increase liver cell viability and reduce ALT activity. Conclusion: Sodium ferulate has a protective effect on liver injury.