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以羊草为试材,采用罗氏454二代测序方法,研究了羊草转录因子家族在逆境胁迫条件下来自38个家族的243个转录因子表达差异模式,以期揭示WRKY、C3HL、NAC等转录因子家族差异基因数量,探讨羊草的抗逆分子机制。结果表明:羊草转录组测序片段经de novo拼接共获得104 105条Unigene序列,对照含有97个转录因子基因,盐碱处理组含有213个转录因子,其中67个转录因子为2组共表达。差异基因表达分析揭示羊草主要通过WRKY、C3HL、NAC转录因子家族的差异表达调控盐碱适应性。该研究为耐盐碱型作物培育奠定了基础。
Using Leymus chinensis 454 second-generation sequencing method, 243 transcription factor expression patterns of Leymus chinensis transcription factor family from 38 families under stress stress conditions were studied in order to reveal the transcription factors WRKY, C3HL, NAC and other transcription factors Family differences in the number of genes to explore the anti-retrograde molecular mechanism of Leymus chinensis. The results showed that 104 105 Unigene sequences were obtained by de novo splicing, 97 of which contained 213 transcription factors and 67 of them were co-expressed in saline-alkali treatment group. Differential gene expression analysis revealed that Leymus chinensis mainly regulates salinity-alkalinity adaptability through differential expression of WRKY, C3HL and NAC transcription factor families. This study lays the foundation for salt-tolerant crop cultivation.