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目的探讨大肠癌血清蛋白质指纹图谱的变化,企以建立诊断模型寻找早期诊断大肠癌的方法。方法利用CM10蛋白芯片和表面增强激光解析电离飞行时间质谱(SELD1-TOF-MS)技术对48例大肠癌患者和34例健康人的蛋白质谱进行分析;运用Ciphergen Protein Chipsoftware5.1软件分析处理数据和筛选标志物,建立蛋白指纹图谱诊断模型。随机另选33例大肠癌患者和34例健康人血清盲法验证大肠癌早期诊断模型。结果(1)48例大肠癌患者与34例健康人血清蛋白质在质荷比(M/N)为2770.34~16061.26间有27个蛋白质含量差异有统计学意义(P<0.05),其中18个M/Z峰在大肠癌中高表达,9个M/Z峰在健康人中高表达。(2)经分析获得由M/Z为2770.34,7971.57,11493.00 3个质谱峰建立的诊断模型,在48例大肠癌患者中有38例被正确分组,34例健康人中32例被正确分组,灵敏度和特异性分别为79.17%(38/48)和94.12%(32/34)。(3)验证研究中经盲法测定灵敏度和特异性分别为72.73%(24/33)和91.18%(31/34)。结论SELD1-TOF-MS技术具有快速、准确、灵敏度和特异性高的优点,通过蛋白芯片仪发现的特异性相关蛋白,有望成为大肠癌早期诊断中有应用价值的临床检测指标。
Objective To explore the changes of serum protein fingerprinting in colorectal cancer and to establish a diagnostic model to find an early diagnosis of colorectal cancer. Methods The protein profiles of 48 patients with colorectal cancer and 34 healthy individuals were analyzed by CM10 protein chip and surface enhanced laser desorption / ionization time of flight mass spectrometry (SELD1-TOF-MS). The data were analyzed by Ciphergen Protein Chipsoftware5.1 software Screening of markers, the establishment of protein fingerprinting diagnostic model. Another 33 patients with colorectal cancer and 34 healthy individuals were randomly selected to confirm the early diagnosis of colorectal cancer by serum blind test. Results (1) The serum protein of 48 patients with colorectal cancer and 34 healthy people had 27 protein contents between 2770.34 and 16061.26 (P <0.05), among which 18 M / Z peak is highly expressed in colorectal cancer, 9 M / Z peak is highly expressed in healthy people. (2) After the analysis, the diagnostic model established by M / Z was 3,770.34,7971.57,11493.00. There were 38 cases in 48 cases of colorectal cancer were correctly grouped, 32 cases of 34 healthy people were correctly grouped, Sensitivity and specificity were 79.17% (38/48) and 94.12% (32/34), respectively. (3) In the validation study, the sensitivity and specificity determined by blinded method were 72.73% (24/33) and 91.18% (31/34), respectively. Conclusion The SELD1-TOF-MS technique has the advantages of fast, accurate, sensitive and specific. It is expected that SELD1-TOF-MS will be a valuable clinical marker in the early diagnosis of colorectal cancer.