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目的:建立测定三七总皂苷传递体中人参皂苷Rg1(Rg1)和人参皂苷Rb1(Rb1)含量和包封率的方法。方法:采用HPLC法测定Rg1和Rb1的含量,测定中色谱柱为Ultimate XB-NH2(4.6 mm×250 mm,5μm),流动相为乙腈-水,采用梯度洗脱,检测波长为203 nm,柱温30℃。以HPLC法结合离心超滤法测定包封率。结果:Rg1和Rb1线性范围分别为0.03472~0.3472、0.03420~0.3420 mg·mL-1(r≥0.999),平均回收率(n=9)分别为100.3%、100.9%;测得PNS传递体中Rg1、Rb1含量分别为3.26、2.60 mg·mL-1,包封率分别为87.9%、98.6%。结论:该法可用于三七总皂苷传递体中Rg1和Rb1的含量测定,方法准确、可靠,专属性强;离心超滤法可用于包封率的测定。
Objective: To establish a method for the determination of ginsenoside Rg1 (Rg1) and ginsenoside Rb1 (Rb1) content and entrapment efficiency in Panax notoginseng sapogenin delivery system. Methods: The content of Rg1 and Rb1 was determined by HPLC. The column was Ultimate XB-NH2 (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile-water. The gradient elution was used. Temperature 30 ℃. Determination of entrapment efficiency by HPLC combined with centrifugal ultrafiltration. Results: The linear ranges of Rg1 and Rb1 were 0.03472 ~ 0.3472,0.03420 ~ 0.3420 mg · mL-1 (r≥0.999), the average recoveries were 100.3% and 100.9%, respectively. The Rg1 , Rb1 contents were 3.26 and 2.60 mg · mL-1, respectively, and the entrapment efficiencies were 87.9% and 98.6% respectively. Conclusion: The method can be used for determination of Rg1 and Rb1 in the total saponins of Panax notoginseng. The method is accurate, reliable and specific. The centrifugal ultrafiltration method can be used to determine the entrapment efficiency.