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提出了一种利用聚焦激光通过单模光纤对酵母细胞驱动的实验方法。基于光压产生光镊的原理,在微通道宽度为100μm的微流控芯片中,波长为650 nm、功率为200 mW的红色半导体激光通过单模光纤对酵母细胞进行驱动。其中有两个关键技术对实验起决定性作用,即激光到光纤的高耦合度和在微流控芯片中以水利聚焦形成单列细胞层流。实验结果表明:在该实验条件下,聚焦激光可以通过单模光纤对酵母细胞进行驱动,且平均速度约为25μm/s,达到了一个较高的驱动速度。该实验方法具有结构简单、检测精确度高及设备成本较低等特点。
An experimental method for driving yeast cells by focusing laser light through a single-mode fiber is proposed. Based on the principle of optical tweezers, a red semiconductor laser with a wavelength of 650 nm and a power of 200 mW was used to drive yeast cells through a single-mode fiber in a microfluidic chip with a microchannel width of 100 μm. Two of these key technologies play a decisive role in the experiment, that is, the high coupling of laser to optical fiber and focusing on water flow in the microfluidic chip to form single cell laminar flow. The experimental results show that under the experimental conditions, the focused laser can drive yeast cells through single-mode fiber, and the average speed is about 25μm / s, reaching a higher driving speed. The experimental method has the advantages of simple structure, high detection accuracy and low equipment cost.