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目的:探讨大鼠幼年时期感染细菌内毒素对其成年后实验性自身免疫性脑脊髓炎(EAE)神经功能损伤和中枢神经系统病理学的影响。方法:取同窝SD幼鼠,于出生后第3和第5天2次给予腹腔注射0.01 mg·mL~(-1)脂多糖(LPS)0.05mL或同等剂量无菌生理盐水。成年后(出生后第8周)用豚鼠脊髓匀浆免疫SD大鼠制作EAE模型,观察并比较这两组大鼠行为学与组织病理学变化。结果:与生理盐水对照组相比,LPS处理组SD大鼠EAE发病率明显降低(P<0.01),且中枢神经系统内炎症细胞浸润及神经髓鞘脱失也明显减轻(P<0.01)。结论:幼年时期感染细菌内毒素对成年SD大鼠EAE有明显的保护作用。
Objective: To investigate the effects of bacterial endotoxin infection in juvenile rats on adult rats with experimental autoimmune encephalomyelitis (EAE) neurological impairment and central nervous system pathology. Methods: The SD littermates were given intraperitoneal injection of 0.01 mg · mL -1 lipopolysaccharide (LPS) 0.05 mL or equivalent sterile saline at 3 and 5 days after birth. Adult (8 weeks after birth) SD rats were immunized with guinea pig spinal cord homogenate to make EAE model. The behavioral and histopathological changes of the two groups were observed and compared. Results: Compared with the saline control group, the incidence of EAE in SD rats was significantly decreased (P <0.01) and the infiltration of inflammatory cells and demyelination in the central nervous system were also significantly reduced (P <0.01). CONCLUSION: Bacterial endotoxin in childhood has obvious protective effect on EAE in adult SD rats.