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目的:对临床常用的酶联免疫吸附法(Enzyme-Liked Immunosorbent Assay,ELISA)检测丙型肝炎的准确性进行分析,为丙型肝炎防治工作提供依据[1]。方法:收集云南省第一人民医院ELISA法检测阳性的69例门诊及住院患者血清,记录患者基本信息及检测结果 OD值,并用重组免疫印迹试验(Recombinant Immunoblot Assay RIBA)作为确证试验对69例标本进行检测。结果:运用重组免疫印迹实验检测ELISA法检测阳性的69例患者血清,其中OD值在0.500-1.000范围内的7例阳性标本经确证阳性2例、阴性4例、不确定性1例,在1.001-1.500范围内的7例阳性标本经确证阳性5例、阴性1例、不确定性1例,在大于1.500范围内的55例阳性标本经确证全为阳性。故在经确证实验检测的69例患者血清中,阳性62例,阴性5例,不确定性2例。结论:ELISA法操作简单,灵敏度高,在临床上筛查丙型肝炎病毒时其真阳性率与OD值大小相关,OD值越大其真阳性率越高,故可对临床检测过程中可疑的标本再运用重组免疫印记实验进行分析以提高检测结果的准确率。
OBJECTIVE: To analyze the accuracy of commonly used enzyme-linked immunosorbent assay (ELISA) in the detection of hepatitis C, and provide evidence for the prevention and control of hepatitis C. [1] Methods: Seventy-nine outpatients and inpatient sera were detected by ELISA in First People’s Hospital of Yunnan Province. The basic information of patients and the OD value of the test results were recorded. Recombinant Immunoblot Assay (RIBA) was used as confirmation test in 69 patients Test. Results: Serum samples from 69 patients with positive immunostaining were detected by ELISA. Among the seven positive samples with OD ranging from 0.500 to 1.000, 2 were positive, 4 were negative, 1 was uncertain, and 1.001 Positive samples in the range of -1.500 were confirmed positive in 5 cases, negative in 1 case and uncertain in 1 case. All the 55 positive samples in the range of more than 1.500 were confirmed as positive. So in the confirmed test of 69 patients serum, 62 cases were positive, negative in 5 cases, 2 cases of uncertainty. Conclusion: ELISA method is simple and sensitive. The true positive rate of screening hepatitis C virus is related to the OD value. The higher the OD value, the higher the true positive rate is. Therefore, the suspicious Specimens and then use recombinant Western blot analysis to improve the accuracy of test results.