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目的:从糖尿病多因素致病角度出发,探讨情志因素对2型糖尿病发病的作用机制———气机失调证,以及具有理气降逆散结功效的复方中药心身5号方的调节作用。方法:采用旋转、拥挤、限制3种情志刺激结合腹腔注射少量链脲佐菌素(STZ)的实验方法,制作了2型糖尿病大鼠模型,实验动物分为正常组、应激组、STZ组、应激+STZ组和中药组。检测血浆去甲肾上腺素(NE)、肾上腺素(E)含量,同时观察心身5号方的调节作用。结果:慢性情志应激可促使STZ鼠血浆去甲肾上腺素(NE)、肾上腺素(E)含量升高,其中肾上腺素在应激+STZ组有统计学意义(P<0.01),中药可降低血浆肾上腺素(E)含量。结论:慢性情志刺激引起实验性2型糖尿病大鼠血浆去甲肾上腺素(NE)、肾上腺素(E)含量升高是糖尿病发病机制之一,同时心身5号方有明显调节作用。
OBJECTIVE: To investigate the mechanism of affective factors on the pathogenesis of type 2 diabetes mellitus (DM) from the perspective of multifactorial diabetes mellitus (T2DM). Methods: A rat model of type 2 diabetes mellitus (T2DM) was established by means of rotating, crowding, and restricting three kinds of emotional stimuli combined with intraperitoneal injection of a small amount of streptozotocin (STZ). The experimental animals were divided into normal group, stress group, STZ Group, stress + STZ group and TCM group. The levels of norepinephrine (NE) and epinephrine (E) in the plasma were detected, and the regulation effect of Xinshitang No.5 was observed. Results: Chronic emotional stress could promote the plasma levels of norepinephrine (NE) and epinephrine (E) in STZ rats. The levels of epinephrine in stress + STZ group were statistically significant (P <0.01) Lower plasma adrenaline (E) content. CONCLUSION: Chronic mood stimulation causes the increase of plasma norepinephrine (NE) and epinephrine (E) in experimental type 2 diabetic rats, which is one of the pathogenesis of diabetes mellitus.