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为探究内生细菌对华山松烂皮病的生防效果及其生理响应,采用平板涂布法和对峙培养法从健康华山松树皮上分离筛选抗烂皮病菌Cenangium ferruginosum的拮抗菌株,运用形态学、生理生化及分子生物学对其进行鉴定;并利用喷雾法研究其对华山松苗烂皮病的盆栽防治效果及苗木各生理指标的变化,观测其对田间发病华山松病斑的防治效果。结果显示,分离得到具有优良拮抗效果的菌株Ba4,经鉴定为解淀粉芽胞杆菌Bacillus amyloliquefaciens;其发酵原液盆栽防治效果可达90.15%,稀释至105防治效果也有17.77%,发病率和病情指数均下降。接种病原菌30 d后,不同浓度Ba4菌剂处理的苗木丙二醛含量均减少,木质素、可溶性蛋白和可溶性糖含量均增加;丙二醛含量与发病率和病情指数呈显著正相关,与防治效果呈显著负相关;木质素、可溶性蛋白和可溶性糖含量则与其相反。田间试验中原液和稀释倍液(10、102和103)的病斑治愈率均超过80%。表明菌株Ba4对松烂皮病有良好的防治效果,能调节华山松抗性物质的合成。
In order to explore the biocontrol effect and physiological response of Endophytic Bacteria to Huashan pine fulva, the antagonistic strains of Cenangium ferruginosum were isolated and isolated from healthy Huashan pine bark by using the plate coating method and the confrontation culture method. Morphological , Physiological and biochemical and molecular biology to identify them; and the use of spray method to study its Huashan pine bark rot disease control effect of potted plants and the physiological indicators of seedlings, to observe the incidence of Huashan pine lesions disease prevention and control. The results showed that strain Ba4 with excellent antagonistic activity was isolated and identified as Bacillus amyloliquefaciens. The control efficacy of the fermented liquid was 90.15% in pot and 17.77% in control with dilution to 105, and the incidence and severity index decreased . After 30 days of pathogen inoculation, the malondialdehyde content of seedlings treated with different concentrations of Ba4 agents decreased, and the content of lignin, soluble protein and soluble sugar increased. The content of malondialdehyde was significantly and positively correlated with the morbidity and disease index, The effect was significantly negatively correlated; lignin, soluble protein and soluble sugar content is the opposite. In the field trials, the cure rates of the spot and dilution times (10, 102 and 103) were over 80%. The results showed that strain Ba4 had a good control effect on loosely stubble disease and could regulate the synthesis of Pinus armandii resistance material.