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AIM:To explore the expression of cadherin isoformsin cultured human gastric carcinoma cells and itsregulation.METHODS:The expressions of cell adhesion molecules(including E-cadherin,N-cadherin,α-caherin,β-catenin)and cadherin transcription factors including snail,slugand twist were determined by reverse transcriptase-polymerase chain reaction(RT-PCR),immunoblottingand immunofluorescence in SV40-immortalized humangastric cell line Ges-1 and human gastric cancer cell linesMGC-803,BGC-823 and SGC-7901.RESULTS:All cell lines expressed N-cadherin,butnot E-cadherin.N-cadherin immunofluorescence wasdetected at cell membranous adherents junctions whereco-localization with immunofluorescent staining ofinner surface adhesion proteins α-and β-catenins wasobserved.The transformed Ges-1 and gastric cancer celllines all expressed transcription factors(snail,slug andtwist)which inhibited the expression of E-cadherin andtriggered epithelial-mesenchymal transformation.CONCLUSION:Cadherin isoforms can change fromE-cadherin to N-cadherin in transformed human gastriccancer cells,which is associated with intracellular eventsof stomach carcinogenesis and high expression ofcorresponding transcription factors.
AIM: To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation. METHODS: The expressions of cell adhesion molecules (including E-cadherin, N-cadherin, α- caherin, β-catenin) and cadherin transcription factors including snail, slugand twist were determined by reverse transcriptase-polymerase chain reaction (RT-PCR), immunoblotting and immunofluorescence in SV40-immortalized humangastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901.RESULTS: All cell lines expressed N-cadherin, butnot E-cadherin. N-cadherin immunofluorescence wasdetected at cell membranous adherents junctions whereco-localization with immunofluorescent staining staining ofinner surface adhesion proteins α-and β-catenins wasobserved.The transformed Ges-1 and gastric cancer celllines all expressed transcription factors (snail, slug andtwist) which inhibited the expression of E-cadherin andtriggered epithelial-mesenchymal transformation. CONCLUSION: Cadherin isoforms c an change from E-cadherin to N-cadherin in transformed human gastriccancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression ofcorresponding transcription factors.