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目的探讨三七总皂苷(PNS)对慢性缺血性肾损伤的保护作用。方法48只SD雄性大鼠随机分为3组:假手术组、模型组和PNS组,每组16只。采用双侧肾动脉不全结扎术建立双侧肾动脉狭窄模型,PNS组于术后开始给予腹腔注射PNS50mg·kg-1·d-1直至处死,其余2组给予同等容量的灭菌注射用水。观察术后14、28、45、60d各组大鼠血细胞间黏附分子1(ICAM-1)、血管紧张素Ⅱ(AngⅡ)水平,肾间质纤维化程度和肾组织α-平滑肌肌动蛋白(α-SMA)的表达。结果与假手术组相比,模型组与PNS组大鼠在各时间点血ICAM-1和AngⅡ水平均增高,肾组织α-SMA的表达也升高,差异均显著(P<0.05)。同时PNS组大鼠各时点血ICAM-1、AngⅡ水平和肾组织α-SMA的表达均低于模型组(P<0.05),且同一时间点病理改变轻于模型组。结论PNS可能通过降低ICAM-1、AngⅡ水平和肾组织α-SMA的表达,延缓肾间质纤维化的进展,进而保护肾功能。
Objective To investigate the protective effect of panax notoginseng saponins (PNS) on chronic ischemic kidney injury. Methods Forty-eight SD male rats were randomly divided into three groups: sham operation group, model group and PNS group, with 16 rats in each group. Bilateral renal artery insufficiency ligation was used to establish a bilateral renal artery stenosis model. The PNS group was given an intraperitoneal injection of PNS 50 mg·kg-1·d-1 until the death. The other two groups were given the same volume of sterile water for injection. The levels of intercellular adhesion molecule-1 (ICAM-1), angiotensin II (AngII), interstitial fibrosis and renal tissue α-smooth muscle actin were observed at 14, 28, 45, and 60 days after operation in rats. α-SMA) expression. Results Compared with the sham-operated group, the levels of ICAM-1 and AngII in blood of the model group and the PNS group increased at each time point, and the expression of α-SMA in the renal tissue also increased, and the difference was significant (P<0.05). At the same time, the levels of ICAM-1 and AngII in blood and the expression of α-SMA in renal tissue at each time point in PNS group were lower than those in model group (P<0.05), and the pathological changes at the same time point were lighter than those in model group. Conclusion PNS may reduce the progression of renal interstitial fibrosis by decreasing the expression of ICAM-1, Ang II and α-SMA in renal tissue, thereby protecting renal function.