论文部分内容阅读
目的：探索一种简单的方法培养大鼠肺动脉内皮细胞（ＲＰＡＥＣｓ）。方法：分离大鼠肺动脉，将其切成小块，使其内皮面贴于培养瓶，用含有２０％新生牛血清、肝素９０μｇ／ｍｌ、Ｌ－谷氨酰胺４ｍｍｏｌ／Ｌ、青霉素１００ｕ／ｍｌ和链霉素１００ｕｇ／ｍｌ的ＲＰＭＩ－１６４０培养基培养。结果：培养２４ｈ后ＲＰＡＥＣｓ从动脉块游出，７２ｈ后将动脉块移去，ＲＰＡＥＣｓ于６～１０ｄ融合成单层细胞。这些细胞具有规律的鹅卵石样形成及Ⅷ因子相关抗原阳性。结论：本方法成功地培养出大鼠肺动脉内皮细胞并通过鉴定。
Objective: To explore a simple method to culture rat pulmonary artery endothelial cells (RPAECs). Methods: The pulmonary arteries of rats were isolated and cut into small pieces. The inner surface of the rat was fixed on the culture flasks and cultured in the medium containing 20% fetal bovine serum, heparin 90μg / ml, L-glutamine 4mmol / L, penicillin 100u / Streptomycin 100ug / ml in RPMI-1640 medium. Results: After cultured for 24h, RPAECs migrated out of the arterial block and removed the arterial block 72h later. The RPAECs fused into monolayers at 6-10 days. These cells have a regular pebble-like appearance and positive for factor Ⅷ related antigen. Conclusion: This method successfully cultured rat pulmonary artery endothelial cells and identified.