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目的 探讨重组人促红细胞生成素(rHEPO)通过小鼠血视网膜屏障的情况及其对视网膜光损伤的保护作用。方法 24只BALB/c小鼠腹腔注射rHEPO,采用酶联免疫吸附测定(ELISA)法检测小鼠视网膜中促红细胞生成素(EPO)的含量; 24只BALB/c小鼠建立光损伤动物模型,通过光镜和核苷酸末端转移酶介导的dUTP缺口标记(TUNEL)法观察实验组小鼠(12只,腹腔注射rHEPO)和对照组小鼠( 12只,腹腔注射生理盐水)视网膜细胞的变化情况。结果 腹腔注射rHEPO后2、4、6及8h小鼠100μg视网膜蛋白中EPO的含量分别为(0 .68±0 .24)、(1 .87±0 .37)、(0. 96±0 .24)及(0. 47±0. 13)mU,差异有统计学意义(F=2 .113,P<0 .05),在腹腔注射药物后4h视网膜中EPO的浓度达到高峰。随光照时间延长,光镜下可见对照组视网膜杆细胞的内、外节破坏明显加重,外核层逐渐变薄,出现核固缩,甚至核碎裂;实验组各观察时间点损伤变化均较轻,仅见感光细胞内、外节排列紊乱,形成空泡,外核层细胞排列稍紊乱,厚度无明显变化。荧光显微镜下观察,对照组视网膜外核层的凋亡细胞随光照时间延长不断增多,至光照后7d凋亡细胞数量减少;实验组视网膜外核层仅见少量凋亡细胞,光照后72h凋亡细胞的数量明显减少,至光照后7d几乎无凋亡细胞。实验组视网膜外核层凋亡细胞的数量?
Objective To investigate the protective effect of recombinant human erythropoietin (rHEPO) on the blood-retinal barrier in mice and its effect on retinal photodamage. Methods Twenty-four BALB / c mice were injected intraperitoneally with rHEPO. The levels of erythropoietin (EPO) in the mouse retina were detected by enzyme-linked immunosorbent assay (ELISA). Twenty-four BALB / c mice were established light- The experimental group mice (12 mice injected intraperitoneally with rHEPO) and the control mice (12 mice injected with saline intraperitoneally) were observed by light microscopy and nucleotidyl transferase mediated dUTP nick-labeling (TUNEL) Changes. Results The levels of EPO in 100 μg of retinal proteins at 2, 4, 6 and 8 h after intraperitoneal injection of rHEPO were (0.68 ± 0.24), (1.87 ± 0.37) and (0.96 ± 0. 24 and 0.47 ± 0.13 mU, respectively. The difference was statistically significant (F = 2.113, P <0.05). The EPO concentration in the retina peaked at 4h after intraperitoneal injection of drugs. With the extension of illumination time, the damage of inner and outer segments of retinal ganglion cells in control group was obviously aggravated by light microscope, and the outer nuclear layer became thinner, nuclear pyknosis and even nuclear fragmentation were observed. In the experimental group, Light, only see photoreceptor cells, the outer part of the disorder, the formation of vacuoles, outer nuclear layer cells arranged a little disorder, no significant change in thickness. Under the fluorescence microscope, apoptotic cells in the outer retinal layer of the control group increased with the prolongation of light time, and the number of apoptotic cells decreased 7 days after light exposure. Only a few apoptotic cells were seen in the outer retinal layer of the experimental group. At 72 hours after light exposure, apoptotic cells The number of significantly reduced, to 7d after light almost no apoptotic cells. Experimental group retinal outer nuclear layer of apoptotic cells?