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背景与目的:鼻咽癌(nasopharygeal carcinoma,NPC)发展至中晚期时,可表现为上行型、下行型或混合型。上行型、下行型具有显著不同的临床特征,治疗策略和预后转归也不同。寻找它们之间的分子差异,对鼻咽癌的分子分型、预后预测和发生发展机理研究具有重要意义。本研究旨在寻找上行型、下行型鼻咽癌之间的差异表达基因。方法:利用涵盖2.13万条基因的寡核苷酸基因芯片(oligo gene chip)技术,筛选上行型、下行型鼻咽癌组织之间的差异表达基因。进一步用逆转录-聚合酶链反应(RT-PCR)方法,分析其中一个差异表达基因在上行型、下行型鼻咽癌组织中的表达情况。结果:用Oligo基因芯片检测分析技术,在上行型、下行型鼻咽癌中发现了17条差异表达基因,其中SELB、Clorf29、GLE1L和FLJ20989基因在上行型鼻咽癌的表达高于下行型鼻咽癌,而1D12A、ASPN、DCN、PRO2219、LRDD、DIO2、ULBP2、PRO3073、IGVH3、IGVH4、IGLJ3、PRO0943和AK057247基因在下行型鼻咽癌的表达高于鼻咽癌上行型;差异倍数在2.30～4.23倍之间。用RT-PCR方法进行研究发现,DIO2基因在下行型鼻咽癌中有90%(9/10)表达较高,而在上行型中只有33.3%(3/9)表达较高,两组表达差异有统计学意义(P=0.020)。结论:上行型、下行型鼻咽癌的基因表达差异有统计学意义;DIO2基因在下行型鼻咽癌的表达高于在上行型,其高表达可能与鼻咽癌的转移潜能密切相关。
BACKGROUND & OBJECTIVE: Nasopharyngeal carcinoma (NPC) may present as upward, downward or mixed type when it develops in the middle and late stages. Up-type, down-type have significantly different clinical features, treatment strategies and prognosis are also different. To find out the molecular differences between them is of great significance for the molecular typing, prognosis prediction and development mechanism of NPC. The aim of this study was to find differentially expressed genes between up and down nasopharyngeal carcinoma. Methods: Using the oligonucleotide microarray technology covering 21, 300 genes to screen differentially expressed genes in up and down nasopharyngeal carcinoma tissues. The expression of one of the differentially expressed genes in up-type and down-type nasopharyngeal carcinoma tissues was further analyzed by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Seventeen differentially expressed genes were found in the up and down nasopharyngeal carcinomas using Oligo microarray assay. The expression of SELB, Clorf29, GLE1L and FLJ20989 in up-type nasopharyngeal carcinoma was higher than that in down-type nasal mucosa Pharyngeal cancer.The expression of 1D12A, ASPN, DCN, PRO2219, LRDD, DIO2, ULBP2, PRO3073, IGVH3, IGVH4, IGLJ3, PRO0943 and AK057247 in NPC was higher than that in NPC ~ 4.23 times between. The results of RT-PCR showed that 90% (9/10) of DIO2 was down-regulated in nasopharyngeal carcinoma and only 33.3% (3/9) was up-regulated in up- The difference was statistically significant (P = 0.020). CONCLUSIONS: The up-regulation and down-regulation of nasopharyngeal carcinoma have significant differences in gene expression. The expression of DIO2 in down-regulation NPC is higher than that in up-regulation, and its high expression may be closely related to the metastatic potential of NPC.