我们应用抗人Ｔ淋巴细胞单克隆抗体ＯＫＴ系列（ＯＫＴ３、ＯＫＴ４、ＯＫＴ８）通过免疫组化（ＡＢＣ法）、体视学定量分析方法，对３２例原发喉鳞状细胞癌组织内浸润的Ｔ淋巴细胞及其亚群进行识别观察和定量分析。结果表明：①ＯＫＴ＋３细胞占喉癌组织内浸润的淋巴细胞的大多数，并且其浸润程度与临床分期有关。②在癌巢间质内ＯＫＴ＋８细胞亚群占优势。ＯＫＴ＋４／ＯＫＴ＋８比值随临床病情的进展而下降甚至倒置。③在喉癌组织内Ｔ淋巴细胞亚群的变化可能与预后有关。 Using OKT3, OKT4, OKT8 monoclonal antibodies against human T lymphocytes, immunohistochemistry (ABC method) and stereological quantitative analysis methods were used to detect the infiltration of T in 32 primary laryngeal squamous cell carcinomas Lymphocytes and their subpopulations were identified and quantitatively analyzed. The results showed that: ①OKT + 3 cells accounted for the majority of infiltrating lymphocytes in laryngeal carcinoma, and the degree of infiltration was related to clinical stage. ② OKT + 8 cell subsets dominated in cancer nests. OKT + 4 / OKT + 8 ratio with the progress of the clinical decline or even upside down. ③ The changes of T lymphocyte subsets in laryngeal carcinoma may be related to the prognosis.