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光肩星天牛(Anoplophora glabripennis)危害多种阔叶树种,曾对“三北”防护林造成重大灾害。花绒寄甲(Dastarcus helophoroides)对光肩星天牛取食不同寄主树如杨树、柳树、榆树和槭树后排出的虫粪表现出不同的趋向性,但对槭树的虫粪未表现出趋向性。为了阐明花绒寄甲成虫差异性趋向选择机制,我们采用动态顶空吸附技术收集不同寄主树的虫粪挥发物,利用气相色谱、固相微萃取-气相色谱-质联仪对虫粪中单萜、倍半萜进行定性定量分析。结果表明,萜烯类是虫粪挥发物的主要成分,且源自不同寄主树木虫粪的萜烯释放量相差显著。检测到的所有化合物中,只有α-古巴烯在源于复叶槭的光肩星天牛虫粪中含量极微,而在源于其他5种寄主树的虫粪中含量均较大。在进一步的Y型嗅觉仪生测实验中,α-古巴烯能够显著地吸引花绒寄甲。α-古巴烯可能是花绒寄甲辨别取食不同寄主树的光肩星天牛虫粪的重要信号化合物。
Anoplophora glabripennis endangers many broadleaf species and has caused major disasters to the “Three Norths” shelterbelt. Dastarcus helophoroides exhibited different tendencies toward feeding insect manure excreted from different host trees such as poplar, willow, elm and maple, but did not show any tendency toward insect pest. In order to elucidate the mechanism of selection of the divergence tendency of adult malachite, we used dynamic headspace adsorption technique to collect the insecticide volatiles of different host trees. We used gas chromatography, solid-phase microextraction-gas chromatography-mass spectrometry Terpene, sesquiterpene qualitative and quantitative analysis. The results showed that terpenes were the main components of pest excretion, and the terpenes released from different host pests had significant differences. Of all the compounds tested, only a-cuba ene was found to be extremely low in Aspergillus auriculae-derived from Acer palmatum, and much larger in insect manure derived from the other five host trees. In a further Y-type olfactometer bioassay experiment, α-CUBE can significantly attract the flocks. α-Cubic ene may be an important signaling compound to identify females that feed on different host trees.