用鸟枪法从耐药质粒ｐＦＣ上克隆到头孢哌酮抗性基因。快速小规模制备质粒ＤＮＡ进行限制性酶切分析鉴定含重组质粒的克隆株，经多次传代，克隆株抗性表达稳定。多种限制性内切酶谱分析并构建重组质粒的物理图谱与质粒ｐＦＣ物理图谱比较，定位出头孢哌酮抗性基因在ｐＦＣ物理图谱上３２ｋｂ至４８ｋｂ区间内，其分子量约１６ｋｂ。该基因包含有ＥｃｏＲⅠ、ＳｍａⅠ、及ＰｖｕⅡ位点。 Cefoperazone resistance gene was cloned from drug-resistant plasmid pFC by shotgun method. Rapid and small-scale preparation of plasmid DNA restriction analysis to identify recombinant plasmids containing clones, after many passages, clone expression of stable. Multiple restriction enzyme analysis and construction of the physical map of the recombinant plasmids compared with the plasmid pFC physical map, locate the cefoperazone resistance gene in the pFC physical map 3  2kb to 4  8kb range, the molecular weight of about 1  6kb. This gene contains the EcoRI, SmaI, and PvuII sites.