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AIM:To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide(P ODN) which can inhibit cerebral thrombosis.METHODS:(1)Oligodeoxynucleotide was synthesized with phosphoramidite method in solid phase,3’ end was modified with thiophosphoter.(2)Uptake and subcellular distribution of P ODN.P ODN was given directly.We observed total uptake rate of P ODN by ECV304 cells and subcellular distribution of P ODN.RESULTS:(1)ECV cells uptake more P ODN with increase of concentration,the uptake rate was about 10.8%-11.9%.(2)Subcellular distribution of P ODN:P ODN was mainly distributed in nucleus especially nucleus sediments(69.3%-77.2%)(P< 0.05).CONCLUSION:P ODN suppressing cerebral thrombosis has favorable transmembrane ability,enzyme resistance and stability.
AIM: To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide (P ODN) which can inhibit cerebral thrombosis. METHODS: (1) Oligodeoxynucleotide was synthesized with phosphoramidite method in solid phase, 3 ’end was modified with thiophosphoter. 2) Uptake and subcellular distribution of P ODN.P ODN was given directly.We observed total uptake rate of P ODN by ECV304 cells and subcellular distribution of P ODN.RESULTS: (1) ECV cells uptake more P ODN with increase of concentration, The uptake rate was about 10.8% -11.9%. (2) Subcellular distribution of P ODN: P ODN was mainly distributed in nucleus especially nucleus sediments (69.3% -77.2% has favorable transmembrane ability, enzyme resistance and stability.