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为了解褐飞虱Nilaparvata lugens(Stl)侵害后水稻耐虫性与植物体内源激素关系,应用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)研究褐飞虱若虫侵害分蘖期超级培矮64S/E32和TN1,灌浆期协优963和TN1后根及叶片脱落酸(abscisic acid,ABA)含量变化。结果表明:褐飞虱侵害分蘖期超级培矮64S/E32和TN1后3d,叶片ABA含量显著上升,ABA含量根冠比(根ABA/叶片ABA)显著下降;侵害后6d,超级培矮64S/E32叶片ABA含量显著下降,根冠比显著上升;但TN1叶片ABA含量在褐飞虱侵害后3d和6d显著上升,根冠比显著下降。褐飞虱侵害灌浆期协优963与分蘖期超级培矮64S/E32变化一致,TN1在褐飞虱侵害后3d叶片ABA含量显著上升,根冠比显著下降;侵害后6d,叶片ABA含量、ABA含量根冠比均显著上升。由ABA含量变化百分比可见,分蘖期ABA含量变化幅度较灌浆期大;耐虫品种变化幅度较感虫品种大,持续期较感虫品种短;叶片变化幅度较根部大。褐飞虱侵害后,两种不同生育期两种抗性不同的水稻品种比较,耐虫品种叶片ABA含量先上升(3d)后下降(6d),ABA含量根冠比先下降(3d)后上升(6d);感虫品种叶片ABA含量持续上升(3d和6d),分蘖期ABA含量根冠比持续下降(3d和6d),灌浆期ABA含量根冠比先下降(3d)后上升(6d);耐、感虫水稻品种根部变化规律不明显。这些差别表明不同水稻(耐虫和感虫)品种受褐飞虱侵害后体内ABA含量变化规律不同。本研究结果对深入阐明水稻耐虫品种的机制具有重要参考价值。
In order to understand the relationship between rice pest resistance and endogenous hormones in plants after Nilaparvata lugens (Stl) infestation, the application of enzyme-linked immunosorbent assay (ELISA) to study the effects of Nilaparvata lugens nymphs infecting tillering stage Pei’aiai 64S / E32 And TN1, and the contents of abscisic acid (ABA) in the roots and leaves of TN1 at the grain filling stage. The results showed that the ABA content of leaves increased significantly at the tillering stage of Pei’ai 64S / E32 and TN1 at tillering stage, and the ABA content root / shoot ratio (ABA / ABA) decreased significantly. On the 6th day after infestation, ABA content decreased significantly and root / shoot ratio increased significantly. However, ABA content in TN1 leaves significantly increased at 3d and 6d after BPH infestation, and the ratio of root to shoot decreased significantly. At the filling stage of BPH, the correlation between the content of ABA and the root / shoot ratio of Xieyou 963 was the same as that of the super Pei’ai 64S / E32 at tillering stage. The ABA content of TN1 significantly decreased and the ratio of root / shoot decreased significantly after 6 days of BPH infestation. All increased significantly. The change of ABA content at tillering stage was larger than that at grain filling stage. The variation range of insect-resistant varieties was larger than that of susceptible varieties and the duration was shorter than that of susceptible varieties. The variation range of leaves was larger than that of roots. The ABA content of the tolerant varieties firstly increased (3d) and then decreased (6d) after the BPH infestation. The root / shoot ABA content decreased first (3d) and then increased (6d) ). The content of ABA in the susceptible varieties continued to increase (3d and 6d), the ABA content at the tillering stage continued to decrease (3d and 6d), and the ABA content decreased first (3d) and then increased (6d) The variation of roots in susceptible rice varieties was not obvious. These differences indicate that the ABA content in different rice (insect-resistant and susceptible) varieties is affected by Nilaparvata lugens (Nilaparvata lugens). The results of this study have important reference value for further elucidating the mechanism of rice pest-resistant varieties.