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以荞麦(Fagopyrum esculentum)为材料,用不同浓度的铝进行土培,研究铝对荞麦根系DNA的损伤效应。结果表明:(1)当铝浓度低于0.45g/kg时,荞麦的总根长增加,根系活力上升,对荞麦生长有一定的促进作用;当铝浓度达到0.6g/kg时,荞麦的根长变短,根系活力下降,不利于荞麦的生长发育。(2)不同浓度铝处理后,荞麦根系DNA呈现出不同程度的增色效应。(3)通过CASP软件对彗星电泳实验图像进行分析显示,随着铝浓度的增加,彗星头部渐小,拖尾逐渐加长。研究结果还表明:铝处理通过对荞麦根系生长产生影响,还对其根系DNA造成损伤,但是从增色效应和彗星电泳的结果可以看出,铝对荞麦所产生的损伤并不是很明显,综合荞麦根系DNA的研究也表明,荞麦是一种耐铝的植物。
Buckwheat (Fagopyrum esculentum) was used as material to study the damage effect of aluminum on the DNA damage of buckwheat roots with different concentrations of aluminum. The results showed that: (1) When the aluminum concentration was lower than 0.45g / kg, the total root length of buckwheat increased and the root activity increased, which promoted the growth of buckwheat. When the aluminum concentration reached 0.6g / kg, Shorter, lower root activity, is not conducive to the growth and development of buckwheat. (2) Different concentrations of aluminum treatment, buckwheat root DNA showed varying degrees of color enhancement effect. (3) By CASP software analysis of comet electrophoresis experimental images show that with the increase of aluminum concentration, comets head gradually smaller, trailing gradually increased. The results also showed that aluminum treatment affected the root growth of buckwheat and damaged its root DNA. However, from the results of enhancement effect and cometary electrophoresis, it can be concluded that the damage of aluminum to buckwheat is not obvious, Root DNA research also shows that buckwheat is an aluminum-tolerant plant.