本文介绍一种适用于临床常规检查,连续显示染色体G、C带型的简便方法。显带试剂只需甲醇—冰乙醇(3:1)固定液、0.025％胰蛋白酶、1/15M磷酸盐缓冲液(pH6.8)和5％Giemsa染液。操作程序极为简便,所显示的G、C带型均符合ISCN(1978)的模式。用此方法对20例患者进行了连续的G、C带型分析,重复性好。对1例1qh+患者和1例inv(9)(p11→q13)患者的同一个分裂相先后作G、C显带分析。经查阅文献,这种用胰蛋白酶显示C带的方法尚未见有报道。 This article describes a suitable routine clinical examination, continuous display of chromosome G, C banding a simple method. The banding reagent only requires methanol-ice ethanol (3: 1) fixative, 0.025% trypsin, 1/15 M phosphate buffer (pH 6.8) and 5% Giemsa dye. The operating procedure is extremely simple and the G and C bands shown are in accordance with the ISCN (1978) model. Twenty patients underwent continuous G-band and C-band analysis with good reproducibility. G, C banding analysis was performed successively on the same cleaved phase of 1 patient with 1qh + and 1 patient with inv (9) (p11 → q13). After reviewing the literature, this method of displaying C-band with trypsin has not been reported yet.