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目的:探讨抑制Bmi1表达对舌鳞癌细胞迁移和侵袭的影响。方法:应用Western免疫印迹检测沉默Bmi1后舌鳞癌细胞株UM1细胞(高转移株,UM1 Control siRNA/UM1 Bmi1 siRNA)中侵袭转移相关基因(SOD2、Slug)及干细胞标志物(ABCG2、Nanog)的表达水平。Transwell试验检测沉默Bmi1后UM1细胞的迁移、侵袭能力。球囊形成和平板克隆实验检测沉默Bmi1后UM1细胞的球囊和克隆形成率。CCK8法检测沉默Bmi1后UM1细胞的增殖能力。采用SPSS17.0软件包对数据进行统计学处理。结果 :转染Bmi1 siRNA,使UM1细胞中Bmi1表达水平下调后,UM1细胞的迁移和侵袭能力显著下降;球囊形成率和克隆形成率也显著低于对照组;增殖能力受到抑制;SOD2、Slug和干细胞标志物ABCG2和Nanog的表达水平显著下降。结论:沉默Bmi1可抑制舌鳞癌细胞的迁移和侵袭。
Objective: To investigate the effect of inhibiting the expression of Bmi1 on the migration and invasion of tongue squamous cell carcinoma. Methods: Western blotting was used to detect the expression of SOD2, Slug and ABCG2 in the squamous cell carcinoma cell line UM1 (UM1 Control siRNA / UM1 Bmi1 siRNA) silenced Bmi1 The expression level. The Transwell assay detected the migration and invasion ability of UM1 cells after silencing Bmi1. Balloon formation and plate cloning experiments were performed to detect the balloon and clone formation rate of UM1 cells after silencing Bmi1. CCK8 assay was used to detect the proliferation of UM1 cells after silencing Bmi1. SPSS17.0 software package for statistical analysis of the data. RESULTS: After transfection of Bmi1 siRNA, the expression of Bmi1 in UM1 cells was down-regulated and the migration and invasion ability of UM1 cells were significantly decreased. The formation rate and formation rate of the balloon were also significantly lower than those of the control group. The proliferation ability was inhibited. The expression of SOD2, Slug And the expression levels of stem cell markers ABCG2 and Nanog were significantly decreased. Conclusion: Silencing Bmi1 can inhibit tongue squamous cell carcinoma cell migration and invasion.