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目的 测定奶芎、山川芎与川芎中总生物碱的含量 ,并以此为指标评价三者的质量。方法 用溴麝香草酚蓝同川芎生物碱形成稳定的有色离子对酸性染料比色法测定总生物碱的含量。结果 在连续三年采集的奶芎、山川芎和川芎样品中 ,总生物碱含量以川芎为最高 ,山川芎次之 ,奶芎最低。结论 以总生物碱为指标 ,测得川芎中生物碱最高 ,质量最佳。为了保证川芎的临床疗效 ,建议使用正品川芎。
Objective To determine the content of total alkaloids in milk thistle, Chuanxiong and Chuanxiong, and use this as an indicator to evaluate the quality of the three. Methods The content of total alkaloids was determined by acid dye colorimetric method with bromothymol blue and chuanxiong alkaloids to form stable colored ions. Results The contents of total alkaloids in Chuanxiong, Chuanxiong and Chuanxiong samples collected for three consecutive years were highest in Chuanxiong, followed by those in Shanchuan, followed by those in milk thistle. Conclusion With the total alkaloid as the index, the alkaloids in Chuanxiong have the highest quality and the best quality. In order to ensure the clinical curative effect of Chuanxiong, it is recommended to use authentic Chuanxiong.