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目的 探讨不同年龄白内障患者房水中淀粉样蛋白(β-amyloid protein,Aβ)含量及其与年龄的相关性.设计实验研究.研究对象行手术治疗的白内障患者210人,平均年龄(72.33±10.35)岁.方法 对所有受试者进行全身及眼科检查,在白内障手术开始前前房穿刺抽取房水,运用ELISA法检测样本中Aβ1-40、Aβ142含量.将所有受试者按年龄分为A组(＜60岁)、B组(60～69岁)、C组(60～79岁)、D组(≥80岁).比较各组房水Aβ1-400、Aβ1-42含量的差别及其与年龄的关系.主要指标房水Aβ1-40和Aβ1-42含量.结果 房水中Aβ1-40蛋白含量在A、B、C、D四个组分别为(80.91±9.33)、(85.81±8.97)、(85.46±11.22)、(82.25±10.41) ng/ml(F=2.399,P=0.069);Aβ42蛋白含量分别为(41.98±7.26)n、(42.46±5.75)、(43.57±5.27)、(42.80±6.04)ng/ml(F=0.719,P=0.543).Aβ1√Aβ1-42在A、B、C、D四个组分别为(1.97±0.34)、(2.04±0.23)、(1.98±0.27)、(1.95±0.33)(F=0.680,P=0.565).结论 不同年龄白内障患者房水中Aβ1-40、Aβ1-42含量以及Aβ1-40/Aβ1-42.2比值随年龄增长无明显改变.患者房水中上述淀粉样蛋白含量发生明显变化,则提示可能存在与淀粉样蛋白代谢障碍相关疾病.“,”Objective To explore the correlation between age and β-amyloid protein (Aβ) content of aqueous humor in patients undergoing cataract surgery at different ages.Design Experimental study.Participants 210 patients with cataract treated by surgery,which mean age was 72.33±10.35 years old.Method All participants underwent systematic and ophthalmic examinations.When cataract surgery,the sample of aqueous humor was extracted with anterior chamber paracentesis.The contents of Aβ1-40,Aβ1-42 were detected with ELISA method.All the subjects were divided by age:group A (＜ 60 years),group B (60-69 years old),group C (60-79 years),group D (≥ 80 years).The differences of contents of Aβ11-40,Aβ1-42 in aqueous humor were compared among different age groups,the relationship between the contents of Aβ1-40,Av1-42 in aqueous humor and age was investigated.Main Outcome Measures The contents of Aβ1-40 and Aβ1-42 in aqueous humor.Results The content of Aβ1-40 in aqueous humor in group A,B,C,D was 80.91±9.33,85.81± 8.97,85.46±11.22,82.25±10.41 ng/ml respectively (F=2.399,P=0.069).The content of Aβ1-42 in aqueous humor in group A,B,C,D was 41.98±7.26,42.46±5.75,43.57±5.27,42.80±6.04 ng/ml (F=0.719,P=0.543).Aβ1-40/Aβ1-42 ratio in group A,B,C,D was 1.97±0.34,2.04±0.23,1.98±0.27,1.95±0.33 (F=0.680,P=0.565).Conclusions There were no significant difference of Aβ1-40,Aβ1-42 and Aβ1-40/Aβ1-42 ratio in aqueous humor of patients with cataract at different ages.The contents of Aβ-amyloid protein in aqueous humor have no obvious change with age.If the Aβ-amyloid protein content in the aqueous humor changes markedly,there may be a disease associated with impaired amyloid metabolism.