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初步研究了噻唑锌在离体条件下对水稻黄单胞杆菌Xanthomonas oryzae pv.oryzae(Xoo)生长的抑制作用,以及其在水稻上防治白叶枯病的生物活性及抗性风险。结果表明,噻唑锌在离体条件下抑制Xoo生长的平均EC50值为(90.17±4.66)μg/mL,且通过紫外光诱导难以获得生长不受影响的抗药性菌株。温室盆栽试验表明,活体条件下噻唑锌对水稻白叶枯病的治疗和保护作用EC50值分别为22.90和52.38μg/mL,其治疗作用显著优于保护作用。从药剂处理稻苗后接种Xoo所形成的病斑上,能够筛选到致病力不受影响的Xoo抗药性突变体,其突变频率为13.3%。交互抗性研究表明,噻唑锌与噻枯唑之间存在交互抗性,但抗药性性状不能稳定遗传。内吸传导性研究表明,噻唑锌能被水稻根部和叶片吸收,且表现为向上传导性。噻唑锌对Xoo的活体抑制活性高于其离体活性,且抗性风险低,适用于防治水稻白叶枯病。
The inhibitory effect of zinc thiazole on the growth of Xanthomonas oryzae pv. Oryzae (Xoo) was studied in vitro and its biological activity and resistance to bacterial blight on rice were also studied. The results showed that the average EC50 value of zinc thiazole inhibiting the growth of Xoo in vitro was (90.17 ± 4.66) μg / mL, and it was difficult to obtain the resistant growth resistant strain by ultraviolet light. Pot experiments in greenhouse showed that the biocontrol effect of thiazole zinc on bacterial leaf blight was 22.90 and 52.38 μg / mL, respectively, and its therapeutic effect was significantly better than that of the protective effect. The Xoo-resistant mutants with unaffected pathogenicity were screened from the inoculated Xoo inoculated rice seedlings and the frequency of mutation was 13.3%. Cross-resistance studies showed that there was interaction resistance between thiazole zinc and thiophene, but the resistant characters could not be inherited stably. Intrinsic conductivity studies have shown that zinc can be absorbed by the roots and leaves of rice, and showed upward conductivity. ZTZ has higher in vivo inhibitory activity against Xoo than its in vitro activity and low resistance to Xoo and is suitable for controlling bacterial blight of rice.