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以牛血块为原料，采用超滤、加热、阴离子交换层析技术制备了牛血SOD。牛血块捣碎液1000ml可得比活为11324U／mg蛋白的SOD32mg，活性回收率为54．7％。以明胶为囊材，乳化法制备了SOD明胶微囊。其最佳制备条件为每1g明胶加入SOD20000U，乳化温度为60℃，乳化时间为5min，转速为250r／min。三批微囊的包封率分别为76.54％、81．32％、75．39％。以制得的SOD明胶微囊给小鼠灌胃，可显著性地抑制巴豆油引起的耳片肿胀。
Using bovine blood clot as raw material, bovine blood SOD was prepared by ultrafiltration, heating and anion exchange chromatography. 1000ml of clotting liquid of bovine blood clotted with a specific activity of 11324U / mg protein SOD32mg, the activity recovery was 54.7%. SOD gelatin microcapsules were prepared by gelatin as the shell material and emulsification method. The best preparation conditions for each 1g of gelatin added SOD20000U, emulsifying temperature of 60 ℃, emulsifying time of 5min, speed of 250r / min. The encapsulation efficiency of three batches of microcapsules were 76.54%, 81.32% and 75.39% respectively. The prepared SOD gelatin microcapsules to mice gavage, can significantly inhibit the swelling of the ear tablets caused by croton oil.