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目的探讨反义STAT3转染鼠恶性黑色素瘤B16细胞后,肿瘤细胞辐射敏感性的变化。方法利用反义寡核苷酸转染B16细胞后,以不同剂量γ射线照射。通过CCK-8试剂盒检测细胞增殖变化,Hoechst33258染色对细胞凋亡作形态学上的观察。用Annexin V/PI复染,流式细胞仪检测细胞早期凋亡率的变化。结果反义STAT3转染后加以γ射线照射,B16细胞的增殖相比于两者单独作用组受到明显抑制,细胞凋亡水平也增加。结论反义STAT3寡核苷酸联合γ射线对B16细胞的增殖抑制和诱导凋亡作用明显增强,提高了鼠黑色素瘤B16细胞的辐射敏感性;表明阻断STAT3蛋白表达可能成为一种新的提高肿瘤辐射敏感性的有效手段。
Objective To investigate the changes of radiosensitivity of tumor cells after transfection of antisense STAT3 into B16 cells of malignant melanoma. Methods Transfection of B16 cells with antisense oligonucleotides was performed with different doses of γ-rays. Cell proliferation was detected by CCK-8 kit, and Morphological observation of apoptosis was performed by Hoechst33258 staining. Annexin V / PI staining and flow cytometry were used to detect the changes of early apoptosis rate. Results After transfection with antisense STAT3, the proliferation of B16 cells was significantly inhibited as compared with that of the other two groups. The apoptosis of B16 cells was also inhibited. Conclusion Antisense STAT3 oligodeoxynucleotide combined with γ-ray can significantly enhance the proliferation and induce apoptosis of B16 cells and increase the radiation sensitivity of mouse melanoma B16 cells. It suggests that blockade of STAT3 protein expression may be a new enhancement Tumor radiation sensitivity of the effective means.