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使用改良盐析法、改良酚/氯仿法、改良碘化钾法和试剂盒法,分别对在-80℃保存1~4年的维吾尔族人群血样提取DNA,分光光度仪检测DNA浓度和纯度,0.8%琼脂糖电泳检测,提取的DNA质量采用单因素方差分析比较。结果表明,改良碘化钾法提取DNA质量最好,其次是试剂盒法、酚/氯仿提取法,盐析法提取质量最差;冻存1~4年的血样提取的DNA浓度之间差异极显著(F=618.011,P=0.000),冻存1~4年的血样提取的DNA纯度之间差异极显著(F=27.090,P=0.000)。该研究基于经济实惠、方便操作,提取DNA质量的高低可以确定改良碘化钾法提取DNA质量最好;血样本冻存(-80℃)超过3年对提取的DNA质量有显著的影响。
DNA was extracted from blood samples of Uygur people who had been preserved at -80 ℃ for 1 to 4 years by modified salting-out method, modified phenol / chloroform method, modified potassium iodide method and kit method. The DNA concentration and purity were detected by spectrophotometer, and the concentration of DNA was 0.8% Agarose gel electrophoresis, DNA quality extracted using one-way ANOVA comparison. The results showed that the quality of DNA extracted by modified potassium iodide was the best, followed by kit method, phenol / chloroform extraction method and salting out method. The difference was significant (P <0.05) F = 618.011, P = 0.000). There was a significant difference (P = 0.000) in the DNA purity of blood samples frozen for 1 to 4 years (F = 27.090, P = 0.000). The research is based on the economical and convenient operation, and the quality of extracted DNA can be determined by the improved potassium iodide method to extract the best DNA quality. Blood samples frozen at -80 ℃ for more than 3 years have a significant impact on the quality of DNA extracted.