自然杀伤(NK)细胞是淋巴细胞中一异质性细胞群,在体外它无须致敏即对靶细胞有广谱的细胞毒作用.NK细胞溶解靶细胞的机制尚未完全清楚,最近人们提出在同适宜诱导细胞接触的NK细胞的上清中,有一种可溶性细胞毒因子——NKCF,它参与NK介导的细胞溶解.干扰素(IFN)和IFN诱导剂可在体内、外刺激自然细胞毒性.IFN似乎可以诱导前体细胞分化为成熟细胞毒效应细胞,并可活化成熟NK细胞.但IFN激活NK细胞的机制仍不清楚.本文证明:人类NK细胞产生NKCF总与IFN-α的产生相关,NKCF的产生和它的溶细胞活性似乎都依赖于IFN-α.首先,作者将去粘附细胞的淋巴细胞用Percoll密度梯度离心获取各种组分,再将 Natural killer (NK) cells are a heterogeneous population of lymphocytes that have a broad spectrum of cytotoxic effects on target cells without the need for sensitization in vitro. The mechanism by which NK cells lyse target cells is not fully understood and has recently been proposed In the supernatant of NK cells suitable for cell-cell induction, there is a soluble cytokine, NKCF, which is involved in NK-mediated lysis, and interferon (IFN) and IFN inducers stimulate natural cytotoxicity in vitro and in vivo .IFN seems to induce precursor cells to differentiate into mature cytotoxic effector cells and activate mature NK cells.But the mechanism of IFN activation of NK cells remains unclear.The paper demonstrates that NKCF production by human NK cells is always associated with the production of IFN- , The production of NKCF and its cytolytic activity seem to be dependent on IFN-alpha.Firstly, the authors obtained lymphocytes from adherent cells by Percoll density gradient centrifugation to obtain various components,