论文部分内容阅读
应用逆行荧光标记与Faglu荧光组化相结合技术,探索了大鼠伏隔核CA能传入纤维的脑干起源。大鼠52只,分三组进行实验。单侧注射组,分别将荧光素PI、DAPI或Bb注入一侧伏隔核后,灌流前作优降宁和L-多巴胺腹腔注射,继用Faglu程序处理切片。结果在同侧的A_(10)、A_9、A_8、A_6、A_2和A_1中,发现为荧光素逆行标记的CA神经元;在对侧的A_(10)、A_9、A_2和A_1中,亦见少量的上述神经元。在以PI-Bb或PI-DAPI配对的双侧注射组,在A_(10)和A_9群中可见少量荧光逆行双标记的CA神经元。表明伏隔核除主要接受同侧脑干A_(10)、A_9、A_8、A_6、A_2和A_1的CA传入纤维外,尚接受对侧A_(10)、A_9、A_2和A_1的少量CA传入纤维。此外,在A_(10)和A_9中,存在着少量向双侧伏隔核发出分叉投射的CA神经元。
The combination of retrograde fluorescence labeling and Faglu fluorescence imaging was used to explore the origin of brainstem in the nucleus accumbens CA. Fifty-two rats were divided into three groups for experiment. Unilateral injection group, fluorescein PI, DAPI or Bb were injected into one side of the nucleus accumbens, prior to perfusion and gavage and L-dopamine intraperitoneal injection, followed by Faglu program for processing sections. RESULTS: CA neurons labeled retrogradely with fluorescein were found in the ipsilateral A_ (10), A_9, A_8, A_6, A_2 and A_1. In the opposite A_ (10), A_9, A_2 and A_1 A small amount of these neurons. In the bilateral injection group paired with PI-Bb or PI-DAPI, a small amount of fluorescent retrograde double-labeled CA neurons were seen in A10 and A9 groups. The results showed that nucleus accumbens still received a small amount of CA on the contralateral A 10, A 9, A 2 and A 1 in addition to CA-afferent fibers that mainly accepted the ipsilateral brainstem A_ (10), A_9, A_8, A_6, A_2 and A_1 Into the fiber. In addition, there was a small amount of CA neurons that cast bifurcated projections to the bilateral nucleus accumbens in A_ (10) and A_9.