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目的 探讨宫颈癌组织中RASSF1A(Ras association domain family1A)抑癌基因启动子甲基化状态及临床意义以及与高危型HPVs感染的关系。方法 甲基化特异性聚合酶链反应(MSP)方法检测39例宫颈鳞癌及12例正常宫颈组织中RASSF1A基因甲基化状态。聚合酶链反应(PCR)方法检测宫颈癌组织中HPV16、18型的感染状况。结果 39 例宫颈癌组织中有11 例可见异常甲基化(28.2%);12例正常宫颈组织均未见甲基化;宫颈癌组HPV感染率为69.2%;RASSF1A基因甲基化在淋巴结转移组(75.0%)高于淋巴结未转移组(9.1 %),P<0.05。RASSF1A甲基化在患者年龄、肿瘤大小、病理组织学分级、临床分期和HPVs感染组之间差异无统计学意义, P>0.05。结论 RASSF1A基因启动子区5′CpG岛的高甲基化是导致RASSF1A基因失活的重要机制,可能参与宫颈癌的发生过程。
Objective To investigate the methylation status of the tumor suppressor gene RASSF1A (Ras association domain family1A) and its clinical significance in cervical cancer and its relationship with high-risk HPV infection. Methods Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation status of RASSF1A gene in 39 cases of cervical squamous cell carcinoma and 12 cases of normal cervical tissue. Polymerase chain reaction (PCR) was used to detect HPV16 and HPV18 infection in cervical cancer. Results Of the 39 cases, 11 cases showed abnormal methylation (28.2%) in cervical cancer tissues, no methylation was found in 12 cases of normal cervical tissues, HPV infection rate was 69.2% in cervical cancer cases, methylation of RASSF1A gene in lymph node metastasis Group (75.0%) was higher than the non-lymph node metastasis group (9.1%), P <0.05. The methylation status of RASSF1A was not significantly different between patients with age, tumor size, histopathological grade, clinical stage and HPV infection group (P> 0.05). Conclusion The hypermethylation of 5’CpG island in the promoter region of RASSF1A gene is an important mechanism of inactivation of RASSF1A gene and may be involved in the carcinogenesis of cervical cancer.