条件性基因打靶是在常规的基因打靶 (基于同源重组原理的基因打靶 )的基础上 ,结合重组酶介导的位点特异性重组技术而发展起来的一种基因打靶新方法。与常规的基因打靶方法相比 ,它对生物基因组的修饰具有时空可控性 ,显示出广阔的应用前景。本文主要就条件性基因打靶的原理、方法、特点及应用前景作一综述 Conditional gene targeting is a new gene targeting method developed on the basis of conventional gene targeting (gene targeting based on the principle of homologous recombination) and recombinase-mediated site-specific recombination. Compared with the conventional gene targeting method, it has the space-time controllability for the modification of the biological genome, and shows a broad application prospect. This article mainly reviews the principles, methods, characteristics and application prospects of conditional gene targeting