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通过交联法和自组装法制备了一种双酶型葡萄糖生物传感器。首先以牛血清白蛋白-戊二醛为交联剂以实现对辣根过氧化物酶(HRP)的固载,再利用凝集素-糖蛋白的识别作用将葡萄糖氧化酶(GOD)分子组装到电极表面,制得双酶型的葡萄糖生物传感器。采用原子力显微镜(AFM)考察了复合膜的性质,同时采用循环伏安法和计时电流法考察了该传感器的电化学行为,研究了该修饰电极对葡萄糖的催化还原作用。在优化的实验条件下,该传感器在3.0×10-6～2.4×10-3mol/L内呈现良好的线性,检测下限为8.2×10-7mol/L。
A bi-enzyme glucose biosensor was prepared by cross-linking method and self-assembly method. Firstly, bovine serum albumin-glutaraldehyde was used as cross-linking agent to immobilize HRP, then GOD molecules were assembled into the recognition of lectin-glycoprotein Electrode surface, the bi-enzyme type glucose biosensor. The properties of the composite membrane were investigated by atomic force microscopy (AFM). The electrochemical behavior of the sensor was investigated by cyclic voltammetry and chronoamperometry. The catalytic reduction of glucose by the modified electrode was studied. Under the optimized experimental conditions, the sensor showed a good linearity in the range of 3.0 × 10-6 ~ 2.4 × 10-3mol / L with the detection limit of 8.2 × 10-7mol / L.