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目的研究CyclinD1在人不同部位脑挫伤组织中表达的变化及其与损伤时间的关系。方法88例脑挫伤标本按损伤后存活时间0.5,1,3,24h和3,7,14,30d分为8个实验组,另以6例非脑挫伤的脑作为对照组,应用CyclinD1免疫组织化学并结合图像分析技术观察CyclinD1的变化。结果脑挫伤后,挫伤灶中央CyclinD1阳性细胞几乎丧失,1h后挫伤灶周围CyclinD1阳性细胞开始增加,3h～30d之间各组挫伤灶周围免疫阳性反应的细胞增加显著,在3h～30d一直维持在较高水平;CyclinD1主要见于小胶质细胞和其它胶质细胞,少数神经元也呈阳性。结论人脑挫伤后,CyclinD1在多种脑细胞内表达,以胶质细胞表达明显,CyclinD1阳性细胞在伤后不久即显著增加,故可作为早期脑损伤的诊断指标。
Objective To study the changes of CyclinD1 expression in different contours of human contusion and its relationship with injury time. Methods 88 cases of brain contusion specimens were divided into 8 experimental groups at 0.5, 1, 3, 24 h and 3, 7, 14 and 30 d after injury, and 6 non-cerebral contusion brains were used as control group. Cyclin D1 immunohistochemistry Chemistry and image analysis techniques to observe the changes of CyclinD1. Results After traumatic brain contusion, there was almost no loss of CyclinD1 positive cells in the contusive area. CyclinD1 positive cells began to increase around contusion lesion 1h later. The number of immunoreactive cells around contusive contusion increased significantly between 3h and 30d Higher level; CyclinD1 mainly found in microglia and other glial cells, a few neurons are also positive. Conclusions CyclinD1 is expressed in many brain cells and expressed in glial cells after human brain contusion. The expression of CyclinD1 positive cells increased significantly shortly after injury, so it can be used as a diagnostic indicator of early brain injury.