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建立了浊点萃取石墨炉原子吸收光谱法测定人血白蛋白样品中超痕量铝的新方法。探讨了溶液酸度、络合剂的种类及浓度、非离子表面活性剂的浓度、平衡时间及温度等因素对浊点萃取过程的影响。以1-(2-吡啶偶氮)-2-萘酚为络合剂,Triton X-114为非离子表面活性剂,浊点萃取分离富集10mL样品溶液,Al(Ⅲ)的富集倍数为34.8,在最佳工作条件下,铝的检出限为0.06μg/L;样品测定的相对标准偏差(n=7)为3.6%,加标回收率95.6%~98.4%。方法适合人血白蛋白样品中超痕量铝的分析测定。
A new method for determination of ultra-trace aluminum in human serum albumin by graphite furnace atomic absorption spectrometry with cloud point extraction was established. The effects of solution acidity, the type and concentration of complexing agent, the concentration of nonionic surfactant, equilibrium time and temperature on the cloud point extraction process were discussed. Triton X-114 was used as a nonionic surfactant with 1 - (2-pyridylazo) -2-naphthol as the complexing agent and 10 mL of sample solution was separated by cloud point extraction. The enrichment factor of Al (Ⅲ) was 34.8. The detection limit of aluminum was 0.06 μg / L under the best working conditions. The relative standard deviation (n = 7) of the sample was 3.6% and the recoveries were 95.6% ~ 98.4%. The method is suitable for the analysis and determination of ultra trace trace aluminum in human serum albumin samples.