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目的观察瞬时转染信号转导与转录激活因子3(STAT3)siRNA后对人胃癌细胞SGC-7901增殖、凋亡与侵袭的影响。方法以人胃癌细胞系SGC-7901培养瞬时转染特异性siRNA的SGC-7901细胞系,应用RT-PCR、MTT、流式细胞术和Transwell体外侵袭实验等检测该siRNA对SGC-7901细胞基因表达、细胞增殖、细胞周期、细胞凋亡及侵袭转移能力的影响。结果 STAT3siRNA转染SGC-7901细胞48 h后,STAT3 mRNA水平下降了73.04%,细胞增殖受到明显抑制,抑制率45.73%;STAT3 siRNA组G0-G1期细胞比例增加21.03%,S期细胞比例减少15.24%,细胞凋亡率升高了6.97%;STAT3 siRNA组细胞侵袭力下降了45.26%(P<0.01)。结论应用siRNA技术能有效抑制SGC-7901 STAT3基因的表达,进而抑制细胞的增殖,诱导细胞凋亡,降低其侵袭力,为以STAT3为靶向的胃癌基因治疗提供了新的思路和手段。
Objective To observe the effects of transient transfection of signal transducer and activator of transcription 3 (STAT3) siRNA on proliferation, apoptosis and invasion of human gastric cancer cell line SGC-7901. Methods SGC-7901 cell line was transiently transfected with human gastric cancer cell line SGC-7901 and the gene expression of SGC-7901 cells was detected by RT-PCR, MTT, flow cytometry and Transwell in vitro invasion assay , Cell proliferation, cell cycle, apoptosis and invasion and metastasis. Results After STAT3 siRNA transfected SGC-7901 cells for 48 h, the level of STAT3 mRNA decreased by 73.04%, and the cell proliferation was inhibited by 45.73%. The percentage of cells in G0-G1 phase increased by 21.03% and that in S phase decreased by 15.24 %, The apoptotic rate increased by 6.97%; the invasiveness of STAT3 siRNA group decreased by 45.26% (P <0.01). Conclusion The siRNA technology can effectively inhibit the expression of STAT3 gene in SGC-7901, and then inhibit the proliferation, induce the apoptosis and decrease the invasiveness of SGC-7901, which provides a new idea and method for the gene therapy of gastric cancer targeting STAT3.