【摘 要】
：
AIM: To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6with the nuclear metrix of carcinoma cells.METHODS:
【机 构】
：
Department of Histology and Embryology，Shantou University Medical College,Tumor Pathology Laboratory
【基金项目】
：
广东省自然科学基金;广东省医药卫生科研项目
论文部分内容阅读
AIM: To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6with the nuclear metrix of carcinoma cells.METHODS: Two esophageal carcinoma cell lines, EC/CUHK1 and EC/CUHK2, were tested for HPV-16 E6subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualizethe expression of E6 subgene in the cells.RESULTS: The HPV-16 E6 subgenetic fragment wes found to be present in nuclear metrix-associeted DNA, E6oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. It was not detected, however, in EC/CUHK1 cells.CONCLUSION: The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma.
其他文献
目的进一步限定壶腹周围肿瘤染色体9p21区域缺失范围。方法选择染色体9p21区域5个微卫星多态性标记，通过聚合酶链反应、聚丙烯酰胺凝胶电泳和银染法，检测35例壶腹周围肿瘤组织
AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer.METHODS:
选用聚酰胺和聚乙烯醇作为皮胶原模型物,分别模拟皮胶原分子中的肽基和羟基,将乙烯基类聚合物/蒙脱土纳米复合鞣剂在不同用量和pH条件下与之作用,以复合鞣剂与胶原模型物的结
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
AIM: To study the anti-hepatofibrosis mechanism of Bie Jie Jian oral liquid (BOL).METHODS: The model was induced by subcutaneous injection of CCl4. BOL was adm
AIM: To evaluate the relationship between the expression of lipopolyaaccharides (LPS) binding protein (LBP) and CD14mRNA and the severity of liver injury in alc
AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD).METHODS: WD coppe
AIM: To investigate FasL expression in hilar cholangiocarcinoma tissues and cultured cholangiocarcinoma cells, and to assess its ability to i
目的探讨转染B7基因的U14疫苗能否在体内诱导中国615系小鼠抗宫颈癌主动免疫应答。方法将小鼠B7-1基因导入615系小鼠宫颈癌U14细胞，建立高效表达B7-1的U14细胞株 (B7+U14) 及
在热力特性、环境安全特性及技术成熟性等方面比较了氨和其他几种工质,介绍了氨在空调制冷领域应用中几项关键技术的发展,以及氨系统目前的应用情况.