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采用分子生物学技术构建了分子克隆文库,并对密云水库水源水中微生物群落分布进行了解析,同时考察了一年内微生物的群落变化,进而评估了出现大规模水介传染病的暴发风险。结果显示,水源水中的细菌群落随着季节变化进行着相对稳定的演变,未出现某种微生物急剧增加或突然暴发的态势,且未发现样品中序列与某种致病菌有直接亲缘关系。因此,该水库水源水发生水介传染病的风险较低。克隆文库分析表明,一年中1月水源水样品的最优势门类为蓝细菌门,所占比例为25.5%;4月蓝细菌门比例上升至39.4%,仍为最大门类;6月、8月、10月水源水中比例最高的为放线菌门,丰度分别为33.8%、35.5%和25.6%。蓝细菌门与放线菌门均为水体中与嗅味相关的细菌群类,应加强对该种微生物的关注,提前做好水厂工艺调整应对预案。
Molecular cloning library was constructed by using molecular biology technique. The distribution of microbial community in Miyun reservoir water source was analyzed. Meanwhile, the community variation of microorganism within one year was examined and the outbreak risk of large-scale water-borne infectious disease was evaluated. The results showed that the bacterial community in the source water changed relatively steadily with the seasons, and no sharp increase or sudden outbreak of some microorganisms appeared. No direct genetic relationship was found between the sequence and some pathogenic bacteria in the samples. As a result, there is a lower risk of water-borne infectious disease in the reservoir source water. Clone library analysis showed that in January of the year, the most predominant species of water source water samples were Cyanobacteria, accounting for 25.5%; in April the proportion of Cyanobacteria rose to 39.4%, still the largest category; June and August The highest proportion of water sources in October was Actinomycetes, with abundances of 33.8%, 35.5% and 25.6%, respectively. Cyanobacteria door and actinomycetes are bacteria groups associated with the smell of water, should pay more attention to the species of microorganisms, water treatment plant to make adjustments in advance to deal with plans.