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以江苏道地药材茅苍术为试材,采用盆栽实验方法,研究不同氮素水平对茅苍术光合特性及生理指标的影响。结果表明:在6个不同氮浓度处理下,11 g·m~(-2)(N)处理的茅苍术叶绿素含量、净光合速率(P_n)、气孔导度(G_s)和蒸腾速率(T_r)较大,胞间二氧化碳浓度(C_i)较小,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性均保持较低水平,且丙二醛(MDA)与游离脯氨酸(Pro)含量都较低。而低氮水平(0、3 g·m~(-2))和高氮水平(20 g·m~(-2))都会不同程度地减弱植株的光合作用,但3种抗氧化酶活性会升高,同时MDA与Pro含量也升高。这说明11 g·m~(-2)是茅苍术生长发育较为合适的氮浓度,而其余处理浓度会影响植物的光合作用,使活性氧积累变多,加速细胞膜脂过氧化作用,细胞膜结构受到损伤,从而影响茅苍术植株正常的生长。
Taking the medicinal plants of Atractylodes macrocephala in Jiangsu Province as test materials, pot experiments were conducted to study the effects of different nitrogen levels on photosynthetic characteristics and physiological indexes of Atractylodes macrocephala. The results showed that chlorophyll content, net photosynthetic rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) of Atractylodes lancea under 11 g · m -2 (N) (C_i) was lower, the activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were kept lower, and the content of malondialdehyde (MDA) ) And free proline (Pro) content is lower. However, low nitrogen (0, 3 g · m -2) and high nitrogen (20 g · m -2) all decreased the photosynthesis of plants to varying degrees, but the activities of three antioxidant enzymes Increase, while MDA and Pro content also increased. The results showed that 11 g · m -2 was the most suitable nitrogen concentration for the growth and development of Atractylodes lancea, while the other concentrations affected the photosynthesis of plants, increased the accumulation of reactive oxygen species, accelerated the lipid peroxidation of the cell membrane, and affected the cell membrane structure Damage, thus affecting the normal growth of plants Atractylodes.