细菌冰核基因的应用研究已成为生物冰核领域的研究热点 ,涉及细菌细胞表面展示、报告基因、病原微生物高敏检测、作物抗寒育种等多个领域 ,显示良好应用前景。在本研究开展前 ,我国尚未克隆到细菌冰核基因 ,因此无法开展冰核基因的应用研究。冰核细菌的促冻杀虫理论确凿无疑 , The application of bacterial ice nucleus gene has become a hot research field in the field of biological ice nucleus, involving many aspects such as bacterial cell surface display, reporter gene, detection of high sensitivity of pathogenic microorganisms, cold breeding of crops and so on, showing good application prospects. Before the research was carried out, there was no bacterial ice nucleus gene cloned in our country, so we could not carry out the application research of ice nucleus gene. Ice-cold bacteria and insecticidal freeze-dried bacteria theory is clear,