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该研究首次成功实现了大鲵在非原产地山东地区的人工繁殖。在催产的14尾大鲵(♀:♂=8:6)中,有4尾产卵,催产率为50%,共产卵2014粒;在催产的6尾♂性中有2尾采集到精液,催产率为33.3%。经人工授精,获得受精卵641枚,受精率约31.8%;建立了微流水孵化技术,孵出稚鲵601尾,孵化率达93.75%。通过对大鲵的成熟度与效应时间、受精率等的相关性探讨,可为我省大鲵养殖业的发展奠定重要基础。
For the first time, this study successfully achieved the artificial breeding of the big raccoon dog in the non-native Shandong area. Among 14 fathers (♀: ♂ = 8: 6) in oxytocin, 4 were spawning with a rate of 50% for co-producing eggs and 2014 spawning. Two of six sexes in the oxytocin were semen collected, The oxytocin rate was 33.3%. After artificial insemination, 641 fertilized eggs were obtained and the fertilization rate was about 31.8%. Microfluidic hatching technology was established and 601 juveniles were hatched with a hatching rate of 93.75%. Through the research on the correlation between the maturity and the effect time and the fertilization rate, we can lay an important foundation for the development of the mackerel breeding industry in our province.