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用过氧化氢、醋酸铜、人血清分别作为羟自由基形成的基质、催化剂，被作用物，以检测过氧化脂质（ＬＰＯ）含量间接反映羟自由基形成量．结果表明，血清中加入同浓度过氧化氢和不同浓度的醋酸铜，其ＬＰＯ量与铜离子浓度呈正比；单加醋酸铜形成少量羟自由基，其ＬＰＯ含量与铜离子浓度也呈正比；单加过氧化氢不形成羟自由基，其ＬＰＯ含量反与过氧化氢浓度呈反比．经同条件过氧化的糖尿病、冠心病、高血压病人血清ＬＰＯ量较正常人血清（经过氧化）明显升高（Ｐ＜０．０１），表明三种病人血清（细胞外）抗氧化能力明显下降，故体内Ｖ．Ｃ、Ｖ．Ｅ、β－胡萝卜素等对提高机体抗氧化能力，降低ＬＰＯ含量是有明显作用的
Hydrogen peroxide, copper acetate and human serum were used as substrate, catalyst and substrate of hydroxyl radical to indirectly reflect the formation of hydroxyl radical in order to detect the content of lipid peroxidation (LPO). The results showed that the concentration of LPO and the concentration of copper ion were proportional to the concentration of copper oxide when the same concentration of hydrogen peroxide and different concentrations of copper acetate were added into the serum. A small amount of hydroxyl free radical was formed by adding copper acetate. The content of LPO was also proportional to the concentration of copper ions. Addition of hydrogen peroxide does not form hydroxyl radicals and its LPO content is inversely proportional to the concentration of hydrogen peroxide. Serum LPO levels in diabetic patients, coronary heart disease and hypertension patients under the same conditions were significantly higher than those in normal people (P <0.01), indicating that the serum (extracellular) antioxidant capacity of the three patients was significantly decreased Therefore, the body V. C, V. E, β-carotene and other enhance the body’s antioxidant capacity, reduce the content of LPO has a significant role