论文部分内容阅读
目的 为明确哪几种膜迷路蛋白成分参与了实验性自身免疫性内耳病 (autoimmuneinner -eardisease ,AIED)的形成。方法 以同种内耳组织抗原免疫豚鼠 ,建立自身免疫性内耳病动物模型。用免疫转印迹技术 (West ernblot)检测动物血液中抗膜迷路蛋白抗体。结果 豚鼠膜迷路蛋白成分复杂 ,SDS -PAGE电泳至少可分出 5种 ,但免疫后大多数动物 ( 15 2 0 )体内仅产生了抗 6 8kD和 136kD膜迷路蛋白抗体 ,而对照组则无阳性反应。结论 膜迷路 6 8kD和 136kD蛋白的免疫反应可能是实验性自身免疫性内耳病的自身免疫基础
The purpose of this study was to determine which types of labyrinthine proteins are involved in the formation of autoimmune inner-ear disease (AIED). Methods Guinea pigs were immunized with the same kind of inner ear tissue antigen to establish an animal model of autoimmune inner ear disease. Anti-lag antibody was detected in the blood of the animals by Western blotting. Results The results showed that the components of guinea pig labyrinthine membrane were complicated. At least 5 kinds of labyrinth cells could be separated by SDS-PAGE, but only anti-6 8 kD and 136 kD labyrinth antibodies were produced in most animals (15 2 0) after immunization, but not in the control group reaction. Conclusions The immune responses of membrane-bound 6 8 kD and 136 kD proteins may be the basis of autoimmunity in experimental autoimmune inner ear disease